Successful research depends on infrastructure of an internationally high standard in the form of experimental equipment, databases, libraries, collections and biobanks.
The following research infrastructures have financial support from the Disciplinary Domain of Medicine and Pharmacy during 2020–2022.
Local research infrastructures
- Array and analysis facility
- Clinical Metabolomics and Therapeutic Drug Monitoring
- Glycosaminoglycan structural analysis
- Hedenstierna translational laboratory
- IMBIM BSL3-Lab
- Low Molecular Mass Spectrometry
- NMR Uppsala
- Preclinical Cancer Treatment (PCT) Center
- Preclinical PET-MRI Platform
- Uppsala Biobank
- Uppsala Clinical Research Center (UCR)
- Uppsala University Behavioral Facility
Research infrastructure at the Disciplinary Domain of Science and Technology
National research infrastructures
Array and analysis facility
The Array and analysis facility provides microarray related services based on the Thermofisher Gene Chip 3000 and Gene Titan systems, which includes analysis of mRNA/ncRNA levels, miRNA levels, DNA copy number measurements and whole genome/custom SNP genotyping etc. In addition we provide bioinformatic support and develop algorithms for problems that many users face.
More information: Array and Analys Facility
BioVis - Platform for Biological Visualisation
The Platform for Biological Visualisation, BioVis, provides a unique combination of technology and know-how for visualization of biological samples at the tissue, cell and subcellular level. We specialise in microscopy, electron microscopy, flow cytometry and image analysis. We can offer state of the art electron-, confocal and light microscopy, as well as analytical and imaging flow cytometry and cell sorting.
The Disciplinary Domain of Medicine and Pharmacy at Uppsala University supports BioVis financially, thus the user fees can be kept low. The platform is available for all universities, governmental agencies and private companies. With proper training, provided by us, you can get almost complete access to the equipment at BioVis.
More information: www.biovis.uu.se/
Preclinical Cancer Treatment (PCT) Center
The Preclinical Cancer Treatment (PCT) Center provides preclinical and clinical researchers with the possibility to evaluate novel drugs in combination with standard treatments in vivo and to refine current cancer therapies. The facility uses medical systems similar to those used in the clinical setting for treating patients and is equipped with both an advanced cone-beam-CT guided X-ray irradiator and a bioluminescence imaging system. The efficacy of irradiation treatment or of e.g. novel targeted therapies can be further evaluated using bioluminescent imaging.
The PCT Center offers multi-fractionated image-guided radiotherapy, help with irradiation treatment planning, and additional support (i.e. chemotherapy, data analysis etc.)
More information: igp.uu.se/infrastructures/preclinical-cancer-treatment/
Hedenstierna translational laboratory
Hedenstierna translational laboratory is one of Scandinavia's most advanced large animal laboratories and is internationally highly renowned. It can perform all kinds of large animal research; advanced surgical interventions in all parts of the body, transplantations, cardiopulmonary bypass, dialysis, respiratory research, all kinds of imaging (e.g., CT-PET and MR-PET) and large animal models for sepsis/antibiotic research and pharmacodynamic and pharmacokinetic studies.
Moreover, basic physiology/pathophysiology as well as model diseases can be studied from the full body and organ perspectives down to single cell reactions. The laboratory is a core facility and has a substantial national and international collaboration.
Contact/More information: Miklós Lipcsey, Department of Surgical Sciences
The major aims of the IMBIM BSL3-Lab are to provide services for design, performance and analyses on BSL3 organisms for internal and external research groups. Our coworkers have a solid experience of research mainly on zoonotic BSL3 viruses, but the facility will be further expanded for studies also on BSL3 bacteria.
The IMBIM BSL3-Lab offers all required experience and equipment for isolation, amplification and characterisation of the microorganisms. Since our BSL3-Lab is the only laboratory that handle human pathogens of BSL3-class, we also serve the hospital with analyses that require BSL3-level.
Clinical metabolomics and therapeutic drug monitoring
The Facility for Clinical metabolomics and Therapeutic Drug Monitoring (Clinical Phenomics) provides high-resolution mass spectrometry services with ThermoFisher Scientific instruments (Q Exactive Orbitrap technology). Our services include analyzes of low molecular weight substances in human body fluids (metabolomics) and the development of therapeutic drug monitoring (TDM) methods. The facility is located at the Department of Medical Sciences at Uppsala University Hospital (Clinical Chemistry and Pharmacology: KKF). The laboratory is accredited by SWEDAC (SS-EN ISO 15189) to deliver results on patient samples and as decision support in clinical trials.
Are you interested in using the facility?
Contact/More information: Kim Kultima, Department of Medical Sciences
Low Molecular Mass Spectrometry
The infrastructure Low Molecular Mass Spectrometry will be run in collaboration with the Clinical Metabolomics and Drug Monitoring (Uppsala University Hospital) and Mass Spectrometry-based Proteomics (Uppsala University, BMC) infrastructures, under a common umbrella. Low molecular mass spectrometry offers services in quantitative and qualitative analysis of small organic molecules with chromatographic technology coupled to mass spectrometry (LC-MS / MS). Tasks include analysis of both endogenous substances and drugs and their metabolites in primarily biological material. Research questions that may be relevant are in metabolomics, lipidomics, pharmacokinetics, drug metabolism etc.
Operations start in spring 2020.
NMR Uppsala is a cross-faculty platform that
- Gives Uppsala University researchers open access to modern NMR spectroscopy.
- Serves as a centre of excellence in NMR at Uppsala University and optimizes the use of resources locally at the University and the connection to national platforms.
- Increases the understanding and use of modern NMR.
The platform provides expertise, services, advice and training to the Uppsala University research community, which benefits from NMR spectroscopy. It comprises 600 and 500 MHz NMR spectrometers equipped with cryoprobes, and gives access to 400 MHz NMR for routine analyses of, for example, organic compounds.
The platform is supported by strategic infrastructure grants from the Disciplinary Domain of Medicine and Pharmacy and Department of Chemistry at the Disciplinary Domain of Science and Technology. Researchers from these entities have priority access at a reduced fee, based on instrument time. This instrumentation provides an excellent local facility for structural determination of compounds, ranging from complex natural products isolated in small amounts to polypeptides and small proteins, as well as the dynamics of these compounds. In addition, the platform allows several other investigations to be carried out, for example studies of protein-ligand interactions and metabolomics.
The steering group consists of:
More information: nmrcenter.kemi.uu.se
The Uppsala PET Centre is well known for its established PET chemistry with a world-wide recognition. A clinical PET/MRI system funded by the Swedish Research Council and installed in Uppsala in October 2014. The integration of PET and MRI is becoming a reality, not only with the new PET/MR equipment, but also by the fact that the installation itself physically connects the PET Centre, MRI and cyclotron facilities located centrally within the Uppsala University Hospital.
The aim of the PET/MRI platform is partly to offer access time for research projects, but also to facilitate the build-up of a unique imaging “biobank”, including subjects recruited from the U-CAN and EpiHealth cohorts. This resource is to be made available to researchers within Sweden. Individual researchers can, in parallel, also apply for access to patient and subject data from the U-CAN and EpiHealth records as well as “imaging biobank” information.
Are you interested in using the facility?
Please contact Anders Lundberg for an application form.
Preclinical PET-MRI Platform
The Preclinical PET-MRI Platform is a fully equipped preclinical laboratory with radiochemistry lab, cell lab and in vivo imaging lab. We offer services ranging from radiolabeling of molecules to preclinical molecular imaging and translational studies.
The platform has 4 modern small animal scanners with modalities including positron emission tomography (PET), single photon emission computed tomography (SPECT), magnetic resonance imaging (MRI) and computed tomography (CT). Typical projects may include tracer development, proof-of-pharmacology, BBB-penetration, dose occupancy, PK/PD studies and dosimetry. The academic prices are substantially subsidized.
More information: www.ilk.uu.se/platforms/ppp_en/
Glycosaminoglycan structural analysis
We determine the concentration and structure (disaccharide composition) of glycosaminoglycans in tissues, body fluids and cultured cells. A few mg of tissue or 106-107 cells is enough to get reliable results. The sensitivity can be considerably improved if glycosaminoglycan metabolically labeled with 35S-sulfate is analyzed. After isolation, the glycosaminoglycans are cleaved with enzymes resulting in the generation of disaccharides which are separated by reverse phase ion pairing (RPIP)-HPLC. The disaccharides are then derivatized with a fluorescent acetamide followed by detection and quantification.
Contact/More information: Lena Kjellén, Department of Medical Biochemistry and Microbiology
Uppsala Biobank is an infrastructure for medical research and a center of competence for biobank related matters. We are a collaboration between Uppsala University and Uppsala University Hospital and represents the only biobank of these two principals. Collections include both research samples and samples intended for medical care and treatment.
Uppsala Biobank offers advice and education on biobank issues as well services for sample management, collection, storage, DNA extraction from whole blood and system support for traceability of samples.
Uppsala Clinical Research Center (UCR)
Uppsala Clinical Research Center (UCR) is a regional, national and international resource for clinical research with all the competence and technology needed to transform innovative ideas into translational research projects, clinical studies and registry-based improvement projects.
More information: www.ucr.uu.se/en/
Uppsala University Behavioral Facility
The Uppsala University Behavioral Facility (UUBF) offers assistance to research groups within and outside Uppsala University with the design, execution, registration and interpretation of behavioral studies in mice, rats and fish. The UUBF staff has extensive experience in conducting research with a focus on interpretation and evaluation of behavior.
UUBF's behavioral departments have equipment for evaluating different types of behaviors and completed protocols (SOPs) for a number of these tests. UUBF also helps to write ethical applications, to program and to statistically process, analyze and interpret studies that have already been carried out. In addition, UUBF runs a BSL-2 facility for working with a variety of virus vectors and holds regular doctoral courses focusing on behavior, such as “How to study behavior in vertebrates with focus on fish and rodents”.
U-PRINT: Uppsala University’s 3D-printing facility within medicine and pharmacy
U-PRINT is dedicated to helping researchers and innovators within medicine and pharmacy to develop new methods, custom research tools and other innovations and technical solutions for use within research, diagnostics and healthcare. We mainly carry out 3D-printing using plastic polymers and composite materials but we can also assist with smaller bioprinting projects.
U-PRINTs goal is to contribute to increased innovation, productivity, quality and competitiveness within medicine and pharmacy at Uppsala University. We prioritize high-quality academic research and we have a clear and transparent pricing model.
We offer complete service when it comes to design and 3D-printing as well as consulting services regarding all aspects of 3D-prining of objects for use within the life sciences.
More information: www.mcb.uu.se/u-print